Astoria-Pacific’s SPOTCHECK Total Galactose test is used to help screen for Galactosemia.
When galactose is ingested by an affected newborn, the galactose-1 phosphate accumulates intracellularly causing blood and urine galactose levels to become elevated. Serum galactose measurements can be used to diagnose and treat Galactosemia.
Total Galactose Continuous Flow System
The SPOTCHECK Analyzer automates the measurement of galactose in the newborn’s blood sample by first hydrolizing galactose-1-phosphate to simple galactose using alkaline phophatase. The total galactose is then oxidized by NAD in the presence of galactose dehydrogenase to form galactonoactone and NADH.
Positive Attributes of Instrumentation and Method:
- Semi-quantitative results
- No operator attendance is required after loading sample plate
- Modular system for easy maintenance
- Large capacity autosampler
- Same day turnaround of results
- Totally automatic – no transfer of sample or reagents from one microtiter plate to another.
- Sample throughput is approximately 90 samples per hour.
- Can be run simultaneously with GALT and Phenylalanine assays
- Prepared reagents are stable for at least 14 days
The SPOTCHECK Total Galactose 50 hour Reagent Kit contains all necessary reagents required for analysis and will provide approximately 50 hours of analyzer run time. The approximate number of actualsamples analyzed per kit is conservatively 2500. All reagents are pre-weighed, color coded, and have a 2 year shelf life (with refrigeration).
- Punch one 1/8″ spots into microtiter filter plate.
- Add buffer for sample elution and shake for 30 minutes.
- Filter samples into round bottom microtiter plate.
- Place the sample plate on the auto-sampler.
- Sampler delivers calibrants, controls and samples to the cartridges
- Online incubation
- Online reagent addition
- Fluorometric detector measures NADPH.